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BIOlabSem 3

BIOCHEMISTRY LAB

BIO 2141

Syllabus

  • 01Qualitative and quantitative analysis of biomolecules (carbohydrates, proteins, lipids, nucleic acids)
  • 02Estimation of carbohydrates by Osazone test
  • 03Reducing sugars analysis using Dinitrosalicylic acid (DNS) method
  • 04Glucose estimation by DNS method and enzymatic (GOD/POD) method
  • 05Starch estimation by Iodine method
  • 06Specific activity of amylase
  • 07Protein estimation by Lowry's and Bradford's methods
  • 08Amino acids estimation by Sorenson's titrimetric method
  • 09Cholesterol estimation by Zak's method
  • 10Spectrophotometric detection of DNA/RNA

References

  • David T Plummer Introduction to Practical Biochemistry. Mc Graw Hill Publication 2017
  • Keith Wilson and Walker Principles and Techniques of Practical Biochemistry. Cambridge University Press 2000
Credits Structure
0Lecture
0Tutorial
3Practical
1Total